The Caspase-4 Antibody (CAB19305) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the caspase-4 protein, this antibody allows for the detection and analysis of this key enzyme in a variety of cell types, making it a versatile tool for studies in immunology, cancer research, and inflammatory conditions.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Caspase-4 Antibody
SKU:
CAB19305
Size:
20μL, 100μL
Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.
Purification Method
Affinity purification
Gene ID
837
RRID
AB_2862638
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide,pH7.3.
Western blot analysis of lysates from SW620 cells, using Caspase-4 Rabbit pAb (CAB19305) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5min.
Western blot analysis of lysates from THP-1 cells, using Caspase-4 Rabbit pAb (CAB19305) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Caspase-4 Rabbit pAb (CAB19305) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
