Description
| Product Name: | Catalase Activity Assay Kit - Fluorometric |
| Product Code: | MAES0003 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, animal tissue |
| Assay Time: | 30 min |
| Instrument: | Fluorescence Microplate Reader |
| Sensitivity: | 0.01 U/L |
| Detection Range: | 0.01-6.51 U/L |
| Recovery Rate: | 92 |
| Inter CV: | 6.8 |
| Inter CV: | 3.4 |
CAT is an enzyme in organism that can efficiently and specifically decompose hydrogen peroxide and is a binding enzyme with iron porphyrin as an auxiliary group. CAT clears hydrogen peroxide in the body and protects cells from the toxicity of H2O2. CAT can also oxidize certain cytotoxic substances, such as formaldehyde, formic acid, phenol and ethanol. According to the difference of catalytic center structure, CAT can be divided into two types, one is iron porphyrin structure, also known as iron porphyrin enzyme, the other contain manganese ion, also known as manganese catalase. CAT is common in breathing organisms. It is mainly found in chloroplasts, mitochondria, endoplasmic reticulum, liver and red blood cells of animals.
Catalase can decompose H2O2 to generate H2O and O2, the residual H2O2 in the detection system react with the fluorescent substance, and the content of residual H2O2 is proportional to the fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm, the catalase activity is inversely proportional to the fluorescence intensity.
| Shelf Life: | 6 Months |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Research Area: | Enzymes, Oxidative stress |
