The Cathepsin H Monoclonal Antibody (CAB23573) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. RRID Gene ID 1512 Swiss Prot Synonym ACC4; ACC5; CPSB; ACC-4; ACC-5; Cathepsin H
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Cathepsin H Monoclonal Antibody
SKU:
CAB23573
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC3082
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACC4, ACC5, CPSB, ACC-4, ACC-5, Cathepsin H
Positive Sample:
A-431, Raji, Mouse lung
Cellular Localization:
Lysosome.
Calculated MW:
37kDa
Observed MW:
28kDa
The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. RRID Gene ID 1512 Swiss Prot Synonym ACC4; ACC5; CPSB; ACC-4; ACC-5; Cathepsin H
Purification Method:
Affinity purification
Gene ID:
1512
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Cathepsin H Rabbit mAb (CAB23573) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from Mouse lung, using Cathepsin H Rabbit mAb (CAB23573) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
