The Caveolin-1 Antibody (CAB1555) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically targets caveolin-1 and is highly reactive with human samples. Validated for use in Western blot applications, the CAV1 Polyclonal Antibody enables precise detection and analysis of caveolin-1 expression in various cell types.Caveolin-1, a critical component of caveolae structures on cell membranes, plays a crucial role in signal transduction, lipid transport, and cellular trafficking. Dysregulation of caveolin-1 has been implicated in various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Caveolin-1 Antibody
SKU:
CAB1555
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.
Purification Method
Affinity purification
Gene ID
857
RRID
AB_2762952
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Caveolin-1 Rabbit pAb (CAB1555) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Caveolin-1 Rabbit pAb (CAB1555) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using Caveolin-1 Rabbit pAb (CAB1555) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Caveolin-1 Rabbit pAb (CAB1555) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human prostate cancer using Caveolin-1 Rabbit pAb (CAB1555) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta using Caveolin-1 Rabbit pAb (CAB1555) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded Mouse lung using Caveolin-1 Rabbit pAb (CAB1555) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
