The CBLL1 Antibody (CAB14932) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot and immunofluorescence applications. By binding to CBLL1, researchers can accurately detect and analyze the protein in various cell types, making it an ideal tool for investigations in cell biology and signal transduction pathways.CBLL1, also known as E3 ubiquitin-protein ligase, plays a vital role in regulating the turnover of proteins within cells, influencing processes such as receptor signaling and protein degradation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CBLL1 Antibody
SKU:
CAB14932
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HAKAI, RNF188, CBLL1
Positive Sample:
Mouse heart, Mouse brain, Mouse lung
Cellular Localization:
Nucleus, Nucleus Speckle, Nucleoplasm.
Calculated MW:
55kDa
Observed MW:
60kDa
This gene encodes an E3 ubiquitin-ligase for the E-cadherin complex and mediates its ubiquitination, endocytosis, and degradation in the lysosomes. The encoded protein contains a RING-finger domain and is also thought to have a role in control of cell proliferation. A related pseudogene has been identified on chromosome X. Alternative splicing results in a non-coding transcript variant.
Purification Method
Affinity purification
Gene ID
79872
RRID
AB_2761814
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CBLL1 Rabbit pAb (CAB14932) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
