The CBS Monoclonal Antibody (CAB11612) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has been rigorously validated for use in multiple applications, including immunohistochemistry, immunofluorescence, and ELISA.CBS is a crucial enzyme in the trans-sulfuration pathway, converting homocysteine to cysteine and regulating the levels of hydrogen sulfide in the body. Dysregulation of CBS activity has been implicated in various diseases, including cardiovascular disorders, neurodegenerative diseases, and cancer.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CBS Monoclonal Antibody
SKU:
CAB11612
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0643
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CBSL, HIP4, CBS
Positive Sample:
HepG2, MCF7
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
61kDa
Observed MW:
61kDa
The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. This gene is a major contributor to cellular hydrogen sulfide production. Multiple alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
875
RRID
AB_2861607
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CBS Rabbit mAb (CAB11612) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using CBS Rabbit mAb (CAB11612) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using CBS Rabbit mAb (CAB11612) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using CBS Rabbit mAb (CAB11612) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
