The CCL5 Polyclonal Antibody (CAB24848) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene is one of several chemokine genes clustered on the q arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immune regulation and inflammatory processes. According to the arrangement of N-terminal cysteine residues in mature peptides, the superfamily is divided into four subfamilies. This chemotactic factor belongs to the CC subfamily and is a chemotactic agent for blood monocytes, memory T helper cells, and eosinophils. It causes basophils to release histamine and activate eosinophils. This cytokine is one of the main HIV inhibitors produced by CD8+cells. It is one of the natural ligands of chemokine receptor chemokine receptor 5 (CCR5), which inhibits in vitro replication of HIV-1 R5 strains using CCR5 as a co receptor. Optional splicing leads to multiple transcriptional variants encoding different isomers.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
CCL5 Polyclonal Antibody
SKU:
CAB24848
Size:
100μL, 20μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
C-C motif chemokine 5
Positive Sample:
Rat lung
Cellular Localization:
Secreted.
Calculated MW:
10kDa
Observed MW:
13kDa
This gene is one of several chemokine genes clustered on the q arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immune regulation and inflammatory processes. According to the arrangement of N-terminal cysteine residues in mature peptides, the superfamily is divided into four subfamilies. This chemotactic factor belongs to the CC subfamily and is a chemotactic agent for blood monocytes, memory T helper cells, and eosinophils. It causes basophils to release histamine and activate eosinophils. This cytokine is one of the main HIV inhibitors produced by CD8+cells. It is one of the natural ligands of chemokine receptor chemokine receptor 5 (CCR5), which inhibits in vitro replication of HIV-1 R5 strains using CCR5 as a co receptor. Optional splicing leads to multiple transcriptional variants encoding different isomers.
Purification Method
Affinity purification
Gene ID
81780
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung using CCL5 Rabbit pAb (CAB24848) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
