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Anti-Bcdin3d Antibody (CAB20720)

SKU:
CAB20720
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
  • Immunofluorescence analysis of NIH/3T3 cells using Bcdin3d Rabbit pAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of MCF7 cells, using Bcdin3d antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
€139 - €419
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Description

Antibody Name:Anti-Bcdin3d Antibody (CAB20720)
Antibody SKU:CAB20720
Antibody Size:50µL, 100µL
Application:Western blotting, Immunofluorescence
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 65-197 of mouse Bcdin3d (NP_083512.2).
Application:Western blotting, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 65-197 of mouse Bcdin3d (NP_083512.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EKRP ILGL DVGC NSGD LSVA LYKH FLSP RDGE TCSG ASRE LRIL CCDI DPVL VERA ERDC PFPE ALTF ITLD IMDQ ESRK VPLS SFLS QFGR SVFD MVFC MSVT MWIH LNHG DRGL CEFL AHVS SLCS YLLV E
Cellular Location:
Calculated MW:32kDa
Observed MW:33KDa
Synonyms:AV138748, 4930556P03Rik
Background:O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His, acting as a capping enzyme by protecting tRNA(His from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145; however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145.
Immunofluorescence analysis of NIH/3T3 cells using Bcdin3d Rabbit pAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using Bcdin3d Rabbit pAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of MCF7 cells, using Bcdin3d antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of MCF7 cells, using Bcdin3d antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

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