The Cyclin B1 Antibody (CAB16038) is a high-quality antibody developed for reliable detection and analysis of target proteins. Cyclin B1 is a key player in the control of cell division and has been implicated in various cellular processes like DNA replication and mitosis. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.By targeting the CCNB1 protein, researchers can effectively detect and analyze its expression in a variety of cell types, making it ideal for investigations in cell biology and cancer research.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cyclin B1 Antibody
SKU:
CAB16038
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.
Purification Method
Affinity purification
Gene ID
891
RRID
AB_2763476
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cyclin B1 Rabbit pAb (CAB16038) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using Cyclin B1 Rabbit pAb (CAB16038) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Cyclin B1 Rabbit pAb (CAB16038) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using Cyclin B1 Rabbit pAb (CAB16038) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using Cyclin B1 Rabbit pAb (CAB16038) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using Cyclin B1 Rabbit pAb (CAB16038) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Cyclin B1 antibody (CAB16038). Western blot was performed from the immunoprecipitate using Cyclin B1 antibody (CAB16038) at a dilution of 1:1000.
