The CCNF Polyclonal Antibody (PAC023646) is a valuable tool for research involving CCNF, a protein associated with cell cycle regulation and cancer development. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. By binding to the CCNF protein, researchers can effectively detect and analyze its expression in various cell types, contributing to advancements in cancer biology and therapeutic development.CCNF, also known as cyclin F, is a critical regulator of cell division and DNA damage response pathways, making it a key player in tumorigenesis and tumor progression.
Its dysregulation has been linked to various types of cancer, highlighting the importance of studying its function and potential as a therapeutic target. The CCNF Polyclonal Antibody provides researchers with a reliable tool for investigating the role of CCNF in cancer biology and exploring its potential as a target for novel anti-cancer therapies.
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin F antibody.
Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using Cyclin F antiobdy. The lane on the left is treated with systhesized peptide.
Western blot analysis of extracts from HeLa cells, using Cyclin F antibody.
Background:
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.
Synonyms:
FBX1; FBXO1; F-box only protein 1;
UniProt Protein Function:
CCNF: Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication. Belongs to the cyclin family. Cyclin AB subfamily.Protein type: Cell cycle regulationChromosomal Location of Human Ortholog: 16p13.3Cellular Component: centriole; SCF ubiquitin ligase complex; nucleusMolecular Function: protein bindingBiological Process: mitosis; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; re-entry into mitotic cell cycle; cell division; protein ubiquitination; placenta development
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified. [provided by RefSeq, Jul 2008]
