The CD109 Monoclonal Antibody (CAB23787) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene. RRID Gene ID 135228 Swiss Prot Synonym CD109; CPAMD7; p180; r150; CD109 antigen
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD109 Monoclonal Antibody
SKU:
CAB23787
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC61642
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD109, CPAMD7, p180, r150, CD109 antigen
Positive Sample:
A-549
Cellular Localization:
Plasma Membrane, Cytosol.
Calculated MW:
74kDa/152kDa/159kDa/161kDa
Observed MW:
190kDa
This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene. RRID Gene ID 135228 Swiss Prot Synonym CD109; CPAMD7; p180; r150; CD109 antigen
Purification Method:
Affinity purification
Gene ID:
135228
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CD109 Rabbit mAb (CAB23787) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC):K-562 Exposure time: 60s.
