The CD109 Monoclonal Antibody (CAB23787) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced through hybridoma technology, is specifically designed for use in immunohistochemistry and flow cytometry applications. It recognizes the CD109 protein with high affinity and specificity, making it ideal for investigating the expression and function of CD109 in different cell types.CD109, also known as a glycosylphosphatidylinositol-anchored glycoprotein, has been implicated in multiple biological pathways, including cell proliferation, differentiation, and migration.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD109 Monoclonal Antibody
SKU:
CAB23787
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC61642
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD109, CPAMD7, p180, r150, CD109 antigen
Positive Sample:
A-549
Cellular Localization:
Plasma Membrane, Cytosol.
Calculated MW:
74kDa/152kDa/159kDa/161kDa
Observed MW:
190kDa
This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
135228
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CD109 Rabbit mAb (CAB23787) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC):K-562 Exposure time: 60s.
