Description
CD117/c-Kit Monoclonal Antibody (CAB22750)
The CD117/c-Kit Monoclonal Antibody (CAB22750) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using advanced monoclonal technology, specifically targets the CD117/c-kit protein in human samples and is validated for use in various applications, including immunohistochemistry and flow cytometry.CD117/c-kit, a receptor tyrosine kinase, plays a key role in cell growth, differentiation, and survival, making it a valuable target for studying various cellular processes and identifying potential therapeutic targets in cancer and other diseases. By targeting the CD117/c-kit protein, researchers can further explore its role in signaling pathways and its potential as a biomarker for various cancers, particularly gastrointestinal stromal tumors (GISTs) where CD117/c-kit mutations are common.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | CD117/c-Kit Monoclonal Antibody |
| SKU: | CAB22750 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Clone Number: | ARC56007 |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | QPSV SPGE PSPP SIHP GKSD LIVR VGDE IRLL CTDP GFVK WTFE ILDE TNEN KQNE WITE KAEA TNTG KYTC TNKH GLSN SIYV FVRD PAKL FLVD RSLY GKED NDTL VRCP LTDP EVTN YSLK GCQG KPLP KDLR FIPD PKAG IMIK SVKR AYHR LCLH CSVD QEGK SVLS EKFI LKVR PAFK AVPV VSVS KASY LLRE GEEF TVTC TIKD VSSS VYST WKRE NSQT KLQE KYNS WHHG DFNY ERQA TLTI SSAR VNDS GVFM CYAN NTFG SANV TTTL EVVD KGFI NIFP MINT TVFV NDGE NVDL IVEY EAFP KPEH QQWI YMNR TFTD KWED YPKS ENES NIRY VSEL HLTR LKGT EGGT YTFL VSNS DVNA AIAF NVYV NTKP EILT YDRL VNGM LQCV AAGF PEPT IDWY FCPG TEQR CSAS VLPV DVQT LNSS GPPF GKLV VQSS IDSS AFKH NGTV ECKA YNDV GKTS AYFN FAFK GNNK EQIH PHT | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | PBT, SCFR, C-Kit, CD117, MASTC, CD117/c-Kit |
| Positive Sample: | HEL |
| Cellular Localization: | Cell Membrane, Cytoplasm, Single-Pass Type I Membrane Protein. |
| Calculated MW: | 110kDa |
| Observed MW: | 150kDa |
This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
| Purification Method | Affinity purification |
| Gene ID | 3815 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from HEL cells, using CD117/c-Kit Rabbit mAb (CAB22750) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s. |
