The CD13 Polyclonal Antibody (CAB23653) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable metalloaminopeptidase activity; peptide binding activity; and zinc ion binding activity. Predicted to be involved in several processes, including negative regulation of renal sodium excretion; peptide catabolic process; and proteolysis. Predicted to act upstream of or within angiogenesis and cell differentiation. Located in external side of plasma membrane. Is expressed in several structures, including alimentary system; brain; metanephros; reproductive system; and sensory organ. Orthologous to human ANPEP (alanyl aminopeptidase, membrane).
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD13 Polyclonal Antibody
SKU:
CAB23653
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Apn, AP-M, AP-N, Cd13, P150, CD13
Positive Sample:
HepG2, Mouse kidney, Rat kidney
Calculated MW:
109kDa
Observed MW:
160kDa
Predicted to enable metalloaminopeptidase activity; peptide binding activity; and zinc ion binding activity. Predicted to be involved in several processes, including negative regulation of renal sodium excretion; peptide catabolic process; and proteolysis. Predicted to act upstream of or within angiogenesis and cell differentiation. Located in external side of plasma membrane. Is expressed in several structures, including alimentary system; brain; metanephros; reproductive system; and sensory organ. Orthologous to human ANPEP (alanyl aminopeptidase, membrane).
Purification Method
Affinity purification
Gene ID
16790
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CD13 Rabbit pAb (CAB23653) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using CD13 Rabbit pAb (CAB23653) at a dilution of 1:900 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using CD13 Rabbit pAb (CAB23653) at a dilution of 1:900 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CD13 Rabbit pAb (CAB23653) at a dilution of 1:900 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
