The CD144 Polyclonal Antibody (CAB24490) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through the immunization of rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the CD144 protein, this antibody allows for the detection and analysis of CD144 expression in various cell types, making it a valuable asset in studies related to vascular biology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
CD144 Polyclonal Antibody
SKU:
CAB24490
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
7B4, Vec, VECD, Cd144, VEcad, VE-Cad, CD144
Positive Sample:
Mouse lung
Calculated MW:
88kDa
Observed MW:
90kDa/120kDa
This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. Mice lacking the encoded protein die in utero due to vascular insufficiency, caused by increased endothelial apoptosis. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 8.
Purification Method
Affinity purification
Gene ID
12562
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse lung, using CD144 Rabbit pAb (CAB24490) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
