The CD146/MCAM Polyclonal Antibody (CAB21488) is a high-quality antibody developed for reliable detection and analysis of target proteins. Involved in glomerular filtration and vascular wound healing. Acts upstream of or within angiogenesis. Located in external side of plasma membrane. Biomarker of chronic obstructive pulmonary disease and uveal melanoma.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
CD146/MCAM Polyclonal Antibody
SKU:
CAB21488
Size:
100μL, 20μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD146, MUC18, HEMCAM, METCAM, MelCAM, CD146/MCAM
Positive Sample:
A375, HUVEC, PC-12
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
72kDa
Observed MW:
120kDa
Involved in glomerular filtration and vascular wound healing. Acts upstream of or within angiogenesis. Located in external side of plasma membrane. Biomarker of chronic obstructive pulmonary disease and uveal melanoma.
Purification Method
Affinity purification
Gene ID
4162
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CD146/MCAM Rabbit pAb (CAB21488) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
