The CD146 Polyclonal Antibody (CAB23669) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity towards human samples and is suitable for use in Western blot assays. By targeting the CD146 protein, researchers can easily detect and analyze its expression in different cell types, making it an ideal tool for investigations in cell biology, oncology, and vascular biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CD146 Polyclonal Antibody
SKU:
CAB23669
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD146, CD149, Muc18, s-endo, 1-gicerin, s-gicerin
Positive Sample:
HeLa, MCF7, Mouse heart
Calculated MW:
71kDa
Observed MW:
120kDa
Predicted to be involved in angiogenesis and positive regulation of cell migration. Predicted to act upstream of or within heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. Predicted to be located in external side of plasma membrane; extracellular space; and nucleus. Predicted to be active in plasma membrane. Is expressed in several structures, including adipose tissue; genitourinary system; gut; nervous system; and respiratory system. Orthologous to human MCAM (melanoma cell adhesion molecule).
Purification Method
Affinity purification
Gene ID
84004
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CD146 Rabbit pAb (CAB23669) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
