The CD152/CTLA-4 Monoclonal Antibody (CAB22865) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced with high specificity and affinity, is ideal for use in various research applications, particularly in the fields of immunology and cancer research.The CD152 (CTLA-4) protein, also known as cytotoxic T-lymphocyte-associated protein 4, functions as a key checkpoint molecule in immune system modulation, specifically inhibiting T cell activation and promoting immune tolerance. Its role in immune regulation makes it a promising target for therapeutic interventions in conditions such as cancer, autoimmune diseases, and inflammatory disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD152/CTLA-4 Monoclonal Antibody
SKU:
CAB22865
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC57390
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
293F transfected with CD152/CTLA-4, Recombinant Human CTLA-4 Protein
Cellular Localization:
Cell Membrane.
Calculated MW:
25kDa
Observed MW:
25kDa(Recombinantprotein)
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Purification Method
Affinity purification
Gene ID
1493
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD152/CTLA-4 using CD152/CTLA-4 Rabbit mAb (CAB22865) at 1:8000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of Recombinant Human CTLA-4/CD152 Protein (RP01044), using CD152/CTLA-4 Rabbit mAb (CAB22865) at 1:8000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 10ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
