The CD248 Antibody (CAB12703) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human CD248 and has been validated for Western blot applications. By binding to the CD248 protein, this antibody enables precise detection and analysis of CD248 expression in various cell types, making it essential for research in vascular biology, tumor microenvironment, and fibrosis.CD248, also known as endosialin, is a key player in promoting angiogenesis and fibroblast activation, processes involved in tissue remodeling and wound healing.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD248 Antibody
SKU:
CAB12703
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TEM1, CD164L1, CD248
Positive Sample:
HeLa, A-375, Mouse spleen, Mouse thymus, Rat thymus
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
81kDa
Observed MW:
81kDa/165kDa
Predicted to enable extracellular matrix binding activity and extracellular matrix protein binding activity. Predicted to be involved in cell migration. Predicted to act upstream of or within several processes, including anatomical structure regression; lymph node development; and positive regulation of endothelial cell apoptotic process. Located in extracellular exosome.
Purification Method
Affinity purification
Gene ID
57124
RRID
AB_2759546
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CD248 Rabbit pAb (CAB12703) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Western blot analysis of various lysates using CD248 Rabbit pAb (CAB12703) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
