The CD275 Polyclonal Antibody (CAB23266) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By specifically binding to the CD275 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in immunology and cancer research.CD275, also referred to as programmed death-ligand 1 (PD-L1), is a critical checkpoint molecule that plays a key role in immune evasion and tolerance. Its involvement in regulating T-cell responses and promoting immune tolerance has made CD275 a promising target for the development of immunotherapies in diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
CD275 Polyclonal Antibody
SKU:
CAB23266
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted to enable identical protein binding activity and signaling receptor binding activity. Involved in positive regulation of interleukin-4 production. Located in external side of plasma membrane. Is integral component of plasma membrane. Is expressed in several structures, including extraembryonic component; gonad; hemolymphoid system gland; liver; and stomach. Orthologous to human ICOSLG (inducible T cell costimulator ligand).
Purification Method
Affinity purification
Gene ID
50723
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse spleen, using CD275 Rabbit pAb (CAB23266) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
