The CD28 Monoclonal Antibody (CAB20346) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
This antibody is validated for use in WB, FC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD28 Monoclonal Antibody
SKU:
CAB20346
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC5082-01
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBFCELISA
Recommended Dilution:
WB
1:500 - 1:1000
FC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Tp44, CD28
Positive Sample:
Jurkat, U-937, Mouse spleen, Mouse thymus, Rat spleen
Cellular Localization:
Cell Surface, Cytosol, External Side Of Plasma Membrane, Immunological Synapse, Plasma Membrane.
Calculated MW:
25kDa
Observed MW:
45kDa
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
940
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CD28 Rabbit mAb (CAB20346) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Flow cytometric analysis of CD28 Rabbit mAb Cocotail (10 μg/mL) in Jurkat cells (Orange) compare to Alexa Fluor 647 conjugated goat anti-rabbit pAb (1:600 dilution) Isotype control (Blue) and non-staining control (Red).
