The CD33 Monoclonal Antibody (CAB22704) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through hybridoma technology, is highly specific to human CD33 and is validated for use in various applications, including flow cytometry and immunohistochemistry.CD33 is known to play a role in regulating the activation and differentiation of myeloid cells, making it a key target for studies in immunology and hematological disorders. Research involving CD33 has focused on its implications in diseases such as acute myeloid leukemia (AML) and Alzheimer's disease, where abnormal expression or function of CD33 has been implicated.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD33 Monoclonal Antibody
SKU:
CAB22704
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC57811
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p67, SIGLEC3, SIGLEC-3, CD33
Positive Sample:
U-937, THP-1
Cellular Localization:
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
40kDa
Observed MW:
70-80kDa
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.
Purification Method
Affinity purification
Gene ID
945
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CD33 Rabbit mAb (CAB22704) at 1:7000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
