The CD34 Monoclonal Antibody (CAB19015) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
This antibody is validated for use in WB, IHC-P, ELISA, mIHC applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD34 Monoclonal Antibody
SKU:
CAB19015
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0219
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISAmIHC
Recommended Dilution:
WB
1:1000 - 1:2000
IHC-P
1:100 - 1:400
mIHC
1:100 - 1:400
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD34, CD34 molecule, GIG3, MORT1
Positive Sample:
bEnd.3, NIH/3T3, TF-1, TF-1 treated with PNGase F, NIH/3T3 treated with PNGase F
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
41 kDa
Observed MW:
80-120 kDa
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
947
RRID
AB_2862507
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from TF-1 cells using CD34 Rabbit mAb (CAB19015) at 1:1000 dilution incubated overnight at 4℃. TF-1 cells were treated with PNGase F (6 U/μL) at 37°C for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Western blot analysis of lysates from NIH/3T3 cells using CD34 Rabbit mAb (CAB19015) at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated with PNGase F (6 U/μL) at 37°C for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
The multiplex IHC analysis on paraffin-embedded Human cervical cancer tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : TROP-2 Rabbit mAb (A20824, 1:8000) with TSA-TYR-520 (Green), CD34 Rabbit mAb (CAB19015, 1:100) with TSA-TYR-570 (Red), and Ki67 Rabbit mAb (A20018, 1:500) with TSA-TYR-690 (Magenta). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.
The multiplex IHC analysis on paraffin-embedded Human breast cancer tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : TROP-2 Rabbit mAb (A20824, 1:8000) with TSA-TYR-520 (Green), CD34 Rabbit mAb (CAB19015, 1:100) with TSA-TYR-570 (Red), and Ki67 Rabbit mAb (A20018, 1:500) with TSA-TYR-690 (Magenta). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.
