The CD3d Polyclonal Antibody (CAB23883) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, sourced from rabbits, exhibits high reactivity towards human samples and is validated for use in Western blot applications. By targeting the CD3D protein, this antibody facilitates the detection and analysis of T lymphocytes in various cell types, making it an essential component of research in immunology and cancer biology.CD3D, a component of the T cell receptor complex, plays a crucial role in the activation and function of T lymphocytes in the immune system.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
CD3d Polyclonal Antibody
SKU:
CAB23883
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
T3d, CD3d
Positive Sample:
Mouse thymus, Mouse spleen
Calculated MW:
19KDa
Observed MW:
20kDa
Predicted to enable identical protein binding activity and transmembrane signaling receptor activity. Involved in positive thymic T cell selection. Part of alpha-beta T cell receptor complex. Is expressed in thymus primordium. Human ortholog(s) of this gene implicated in immunodeficiency 19 and severe combined immunodeficiency. Orthologous to human CD3D (CD3d molecule).
Purification Method
Affinity purification
Gene ID
12500
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CD3d Rabbit pAb (CAB23883) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
