The CD42a Polyclonal Antibody (CAB22234) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody is raised in rabbits and has been validated for use in Western blot applications, detecting the CD42A protein in human samples with high specificity.CD42A, also known as glycoprotein Ib alpha, plays a crucial role in platelet function and is essential for normal hemostasis. Dysregulation of CD42A has been implicated in various platelet disorders, making it a promising target for research into thrombotic and bleeding disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD42a Polyclonal Antibody
SKU:
CAB22234
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GPIX, CD42a
Positive Sample:
HEL
Cellular Localization:
Plasma Membrane.
Calculated MW:
19kDa
Observed MW:
19kDa
This gene encodes a small membrane glycoprotein found on the surface of human platelets. It forms a 1-to-1 noncovalent complex with glycoprotein Ib, a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor. The complete receptor complex includes noncovalent association of the alpha and beta subunits with the protein encoded by this gene and platelet glycoprotein V. Defects in this gene are a cause of Bernard-Soulier syndrome, also known as giant platelet disease. These patients have unusually large platelets and have a clinical bleeding tendency.
Purification Method
Affinity purification
Gene ID
2815
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HEL cells, using CD42a Rabbit pAb (CAB22234) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
