The CD55 Monoclonal Antibody (CAB11283) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for CD55 in human samples, making it an excellent choice for applications such as Western blotting and immunohistochemistry.CD55, also called decay accelerating factor (DAF), is involved in preventing the formation of the membrane attack complex (MAC) and promoting the decay of C3 and C5 convertases, ultimately guarding cells against complement-mediated lysis.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD55 Monoclonal Antibody
SKU:
CAB11283
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0568
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CR, TC, DAF, CROM, CHAPLE, CD55
Positive Sample:
HepG2, A-549
Cellular Localization:
Cell Membrane, Gpi-Anchor, Lipid-Anchor, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
41kDa
Observed MW:
78kDa
This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
Purification Method
Affinity purification
Gene ID
1604
RRID
AB_2861540
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CD55 Rabbit mAb (CAB11283) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Human placenta using CD55 Rabbit mAb (CAB11283) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
