The CD69 Monoclonal Antibody (CAB22171) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. RRID Gene ID 969 Swiss Prot Synonym AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26; CD69
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
CD69 Monoclonal Antibody
SKU:
CAB22171
Size:
100μL, 20μL
Reactivity:
Rat
Clone Number:
ARC56158
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AIM, EA1, MLR-3, CLEC2C, GP32/28, BL-AC/P26, CD69
Positive Sample:
Rat spleen
Cellular Localization:
Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
23kDa
Observed MW:
25-45kDa
This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. RRID Gene ID 969 Swiss Prot Synonym AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26; CD69
Purification Method:
Affinity purification
Gene ID:
969
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat spleen, using CD69 Rabbit mAb (CAB22171) at1:4000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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