The CD83 Monoclonal Antibody (CAB22379) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD83 Monoclonal Antibody
SKU:
CAB22379
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC53759
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:3000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BL11, HB15, CD83
Positive Sample:
Daudi, Raji, HeLa
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
23 kDa
Observed MW:
23-65 kDa
The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
9308
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CD83 Rabbit mAb (CAB22379) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
![PE Anti-Human CD83 Antibody [HB15e] (AGEL0542)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/21073/607135/pe-anti-human-cd83-antibody-hb15e-agel0542__15652.1707500224.jpg?c=2 "PE Anti-Human CD83 Antibody [HB15e] (AGEL0542)")
![APC Anti-Human CD83 Antibody [HB15e] (AGEL0543)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/21074/606306/apc-anti-human-cd83-antibody-hb15e-agel0543__22792.1707497591.jpg?c=2 "APC Anti-Human CD83 Antibody [HB15e] (AGEL0543)")
![GenieFluor 488 Anti-Human CD83 Antibody [HB15e] (AGEL2143)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/229603/605003/geniefluor-488-anti-human-cd83-antibody-hb15e-agel2143__81755.1706889048.jpg?c=2 "GenieFluor 488 Anti-Human CD83 Antibody [HB15e] (AGEL2143)")