The CD8A Polyclonal Antibody (CAB22114) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for use in Western blot applications. By targeting the CD8a protein, this antibody facilitates the detection and analysis of CD8a expression in various cell types, making it an essential component in studies related to immunology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
CD8A Polyclonal Antibody
SKU:
CAB22114
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Ly-2, Ly-B, Ly-35, Lyt-2, CD8A
Positive Sample:
Mouse spleen, RAW 264.7
Cellular Localization:
Cell Surface, External Side Of Plasma Membrane, Plasma Membrane, Plasma Membrane Raft.
Calculated MW:
27kDa
Observed MW:
35-42kDa
Enables identical protein binding activity. Acts upstream of or within several processes, including cytotoxic T cell differentiation; defense response to virus; and positive regulation of calcium-mediated signaling. Located in external side of plasma membrane. Is expressed in several structures, including endocrine gland; exocrine gland; genitourinary system; gut; and hemolymphoid system gland. Orthologous to human CD8A (CD8a molecule).
Purification Method
Affinity purification
Gene ID
12525
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CD8A Rabbit pAb (CAB22114) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
