The CD8B Antibody (CAB3286) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in immunology and cancer research studies.CD8b is a key component of the immune system, playing a crucial role in the activation and function of cytotoxic T cells which are critical for eliminating infected or cancerous cells. By targeting CD8b, researchers can accurately detect and analyze the presence of cytotoxic T cells in various cell types, providing insights into immune responses and potential therapeutic targets.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD8B Antibody
SKU:
CAB3286
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LY3, P37, LEU2, LYT3, Ly-3, CD8B1, CD8beta, CD8B
Positive Sample:
293F transfected with CD8B
Cellular Localization:
Cell Membrane, Secreted, Single-Pass Type I Membrane Protein, Single-Pass Type I Membrane Protein.
Calculated MW:
24kDa
Observed MW:
23kDa
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.
Purification Method
Affinity purification
Gene ID
926
RRID
AB_2765026
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD8B using CD8B Rabbit pAb (CAB3286) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
