The CDC20 Antibody (CAB1231) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody is raised in rabbits and has high reactivity with human samples, making it a reliable tool for Western blot applications. By binding to the CDC20 protein, this antibody enables the detection and analysis of CDC20 in various cell types, making it suitable for studies in cell biology and cancer research.CDC20 is a key player in the cell cycle, specifically in the regulation of cell division and chromosome segregation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CDC20 Antibody
SKU:
CAB1231
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cytoplasm, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle Pole.
Calculated MW:
55kDa
Observed MW:
55kDa
CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation.
Purification Method
Affinity purification
Gene ID
991
RRID
AB_2759164
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CDC20 Rabbit pAb (CAB1231) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of L929 cells using CDC20 Rabbit pAb (CAB1231) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
