The CDC20 Antibody (CAB15656) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for CDC20 and is validated for use in Western blot applications. By binding to the CDC20 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential tool for studies in cell biology and cancer research.CDC20 is essential for proper cell cycle progression, as it coordinates the separation of sister chromatids during mitosis. Dysregulation of CDC20 has been linked to chromosomal instability and tumorigenesis, making it a promising target for cancer therapy.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CDC20 Antibody
SKU:
CAB15656
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cytoplasm, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle Pole.
Calculated MW:
55kDa
Observed MW:
55kDa
CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation.
Purification Method
Affinity purification
Gene ID
991
RRID
AB_2763063
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from PC-3 cells, using CDC20 Rabbit pAb (CAB15656) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using CDC20 Rabbit pAb (CAB15656) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using CDC20 Rabbit pAb (CAB15656) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunoprecipitation analysis of 200 μg extracts of LO2 cells using 3 μg CDC20 antibody (CAB15656). Western blot was performed from the immunoprecipitate using CDC20 antibody (CAB15656) at a dilution of 1:1000.
