The CDC25C Antibody (CAB1672) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards CDC25C protein in human samples, making it an ideal choice for Western blot applications.CDC25C, a phosphatase enzyme, plays a crucial role in promoting cell division by activating cyclin-dependent kinases. Dysregulation of CDC25C has been associated with various diseases, including cancer, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CDC25C Antibody
SKU:
CAB1672
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDC25, PPP1R60, CDC25C
Positive Sample:
Mouse lung, Mouse thymus, Rat thymus
Cellular Localization:
Nucleus.
Calculated MW:
53kDa
Observed MW:
60kDa
This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.
Purification Method
Affinity purification
Gene ID
995
RRID
AB_2763727
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CDC25C Rabbit pAb (CAB1672) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
