The CDK5RAP1 Antibody (CAB15844) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and reactivity towards human samples, making it reliable for use in Western blot assays.CDK5RAP1 is known to play a crucial role in centrosome duplication and cell division, making it a promising target for cancer research and drug development. By binding to CDK5RAP1, this antibody facilitates the detection and analysis of the protein in various cell types, enabling a deeper understanding of its function and potential therapeutic applications.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CDK5RAP1 Antibody
SKU:
CAB15844
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
C42, CGI-05, HSPC167, C20orf34, CDK5RAP1
Positive Sample:
A-549, Mouse thymus
Cellular Localization:
Cytosol, Mitochondrion.
Calculated MW:
68kDa
Observed MW:
68kDa
This gene encodes a regulator of cyclin-dependent kinase 5 activity. This protein has also been reported to modify RNA by adding a methylthio-group and may thus have a dual function as an RNA methylthiotransferase and as an inhibitor of cyclin-dependent kinase 5 activity. Alternative splicing results in multiple transcript variants that encode different isoforms.
Purification Method
Affinity purification
Gene ID
51654
RRID
AB_2763270
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CDK5RAP1 Rabbit pAb (CAB15844) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat liver using CDK5RAP1 Rabbit pAb (CAB15844) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using CDK5RAP1 Rabbit pAb (CAB15844) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
