The CDK6 Monoclonal Antibody (CAB0106) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits exceptional reactivity with human samples and has been validated for use in Western blot, immunohistochemistry, and flow cytometry applications.CDK6 is a crucial player in the regulation of cell division and is often dysregulated in cancer, making it a promising therapeutic target for cancer treatment. By targeting CDK6, researchers can better understand the mechanisms underlying cell cycle control and potentially develop new strategies for cancer therapy.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
CDK6 Monoclonal Antibody
SKU:
CAB0106
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC0224
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MCPH12, PLSTIRE, CDK6
Positive Sample:
Jurkat, C6, HeLa
Cellular Localization:
Cell Projection, Cytoplasm, Nucleus, Centrosome, Cytoskeleton, Microtubule Organizing Center, Ruffle.
Calculated MW:
37kDa
Observed MW:
37kDa
The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly.
Purification Method
Affinity purification
Gene ID
1021
RRID
AB_2861450
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CDK6 Rabbit mAb (CAB0106) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
