The CDK9 Monoclonal Antibody (CAB11145) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is validated for use in various applications, including Western blot, immunohistochemistry, and immunofluorescence.CDK9 is a crucial component of the positive transcription elongation factor b (P-TEFb) complex, which plays a critical role in the regulation of RNA polymerase II-mediated transcription. This complex is involved in the expression of many genes, including those involved in cell growth, differentiation, and apoptosis.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CDK9 Monoclonal Antibody
SKU:
CAB11145
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0527
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TAK, C-2k, CTK1, CDC2L4, PITALRE, K9
Positive Sample:
HeLa, 293T , MCF7, Mouse brain, Rat testis
Cellular Localization:
Cytoplasm, Nucleus, Pml Body.
Calculated MW:
43kDa
Observed MW:
42kDa/55kDa
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
Purification Method
Affinity purification
Gene ID
1025
RRID
AB_2861506
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CDK9 Rabbit mAb (CAB11145) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Western blot analysis of lysates from wild type(WT) and CDK9 knockout (KO) 293T cells, using [KO Validated] CDK9 Rabbit mAb (CAB11145) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] CDK9 Rabbit mAb (CAB11145) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] CDK9 Rabbit mAb (CAB11145) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KO Validated] CDK9 Rabbit mAb (CAB11145) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KO Validated] CDK9 Rabbit mAb (CAB11145) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg CDK9 antibody (CAB11145). Western blot was performed from the immunoprecipitate using CDK9 antibody (CAB11145) at a dilution of 1:1000.
