The CDKN2A Polyclonal Antibody (CAB11058) offered by Assay Genie is a crucial tool for researchers studying the CDKN2A protein, also known as p16INK4a, which plays a key role in regulating cell cycle progression and suppressing tumor growth. This antibody, produced in rabbits, exhibits high specificity towards human samples and has been validated for use in Western blot experiments.The CDKN2A Polyclonal Antibody binds specifically to the CDKN2A protein, allowing for accurate detection and analysis in various cell types. Its utility in immunology and cancer research makes it an essential reagent for investigating the mechanisms underlying cell cycle regulation and tumor suppression.
Given the pivotal role of CDKN2A in controlling cell proliferation and preventing tumor formation, this antibody is invaluable for studies on cancer development, progression, and potential therapeutic targets. By providing researchers with a reliable tool to investigate CDKN2A expression and function, the CAB11058 antibody enables advancements in understanding the molecular pathways involved in cancer biology and the development of targeted treatments.
Product Name:
CDKN2A/p16INK4a Rabbit Polyclonal Antibody
SKU:
CAB11058
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-156 of human CDKN2A/p16INK4a (NP_000068.1).
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
Purification Method:
Affinity purification
Gene ID:
1029
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using [KO Validated] CDKN2A/p16INK4a Rabbit pAb (CAB11058) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
