The CDX2 Antibody (CAB1629) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been rigorously validated for use in Western blot applications. By binding specifically to the CDX2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential tool for studies in developmental biology, gastrointestinal diseases, and cancer research.CDX2 is known for its involvement in intestinal cell differentiation, with dysregulation of its expression linked to various diseases, including colorectal cancer and inflammatory bowel diseases.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CDX2 Antibody
SKU:
CAB1629
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDX3, CDX-3, CDX2/AS, CDX2
Positive Sample:
SW620, MCF7, 293T
Cellular Localization:
Nucleus.
Calculated MW:
34kDa
Observed MW:
36kDa/38kDa
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis.
Purification Method
Affinity purification
Gene ID
1045
RRID
AB_2763689
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and CDX2 knockdown (KD) 293T cells using CDX2 Rabbit pAb (CAB1629) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of Human colon cancer cells using CDX2 Rabbit pAb (CAB1629) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat rectum cells using CDX2 Rabbit pAb (CAB1629) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
