The CENPC Antibody (CAB6557) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the CENPC protein, researchers can easily detect and analyze this critical protein in a variety of cell types.CENPC is essential for proper chromosome alignment and segregation during cell division, making it a crucial target for studying cancer biology and genetic disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CENPC Antibody
SKU:
CAB6557
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MIF2, hcp-4, CENP-C, CENPC1, CENPC
Positive Sample:
HeLa, A-431, HepG2, Mouse brain, Rat brain
Cellular Localization:
Chromosome, Nucleus, Centromere, Kinetochore.
Calculated MW:
107kDa
Observed MW:
130kDa
Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12.
Purification Method
Affinity purification
Gene ID
1060
RRID
AB_2767151
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CENPC Rabbit pAb (CAB6557) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
