The CENPM Polyclonal Antibody (PACO16009) is an essential tool for research involving the centromere protein M (CENPM), a key player in chromosome segregation during cell division. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in a variety of applications, including Western blotting.CENPM is crucial for the proper organization and function of the centromere, the region of a chromosome where the kinetochore assembles to ensure accurate segregation of DNA during cell division. Dysregulation of CENPM has been implicated in chromosomal instability, a hallmark of cancer cells.
By targeting CENPM with this antibody, researchers can investigate its role in cell division processes and its potential as a therapeutic target for cancer treatment.Furthermore, studying CENPM using this antibody can provide valuable insights into the mechanisms underlying chromosome segregation errors, which are associated with various genetic disorders and developmental abnormalities. This antibody is a powerful tool for researchers in the fields of cell biology, genetics, and cancer research looking to unravel the complexities of chromosome segregation and its implications for human health.
Antibody Name:
CENPM Antibody (PACO16009)
Antibody SKU:
PACO16009
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human
Immunogen:
Full length fusion protein
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO16009(CENPM Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO16009(CENPM Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
Background:
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).
Synonyms:
centromere protein M
UniProt Protein Function:
CENPM: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. 3 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 22q13.2Cellular Component: nucleoplasm; cytosolBiological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; mitotic cell cycle
UniProt Protein Details:
NCBI Summary:
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008]
centromere protein M; interphase centromere complex protein 39; proliferation associated nuclear element 1; proliferation-associated nuclear element protein 1
UniProt Protein Name:
Centromere protein M
UniProt Synonym Protein Names:
Interphase centromere complex protein 39; Proliferation-associated nuclear element protein 1
