The CENPV Antibody (PAC020280) is a valuable tool for researchers studying the centromere protein V (CENPV), a key player in chromosome segregation during cell division. This polyclonal antibody, generated in rabbits, has high specificity for human samples and has been validated for use in Western blot applications.CENPV is essential for proper cell division and chromosome alignment, making it a crucial target for studies in cell biology and cancer research. By binding to the CENPV protein, this antibody enables researchers to detect and analyze its expression in various cell types, providing insights into its function and potential roles in disease development.
Understanding the role of CENPV in cellular processes is vital for advancing our knowledge of cell division mechanisms and the implications for diseases such as cancer. This antibody is a valuable tool for investigating CENPV function and its potential as a therapeutic target in various research contexts.
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO20280(CENPV Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200).
Gel: 10%SDS-PAGE, Lysate: 40 µg, Lane: Jurkat cells, Primary antibody: PACO20280(CENPV Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second.
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using PACO20280(CENPV Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
CENPV orthologs were detected in a range of species, including plants, but the proline-rich region was detected only in primates, rodents, birds, and amphibians, and long proline stretches were found only in primates. Northern blot analysis showed ubiquitous Cenpv expression in mouse tissues, with highest expression in testis, followed by small intestine, brain, and kidney. Immunofluorescence microscopy of mouse fibroblasts revealed colocalization of Cenpv with stabilized cytoplasmic microtubules. Western blot analysis of Ramos B cells detectedCENPV at an apparent molecular mass of 40 kD.
Synonyms:
centromere protein V
UniProt Protein Function:
CENPV: Required for distribution of pericentromeric heterochromatin in interphase nuclei and for centromere formation and organization, chromosome alignment and cytokinesis. Belongs to the gfa family. 3 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 17p11.2Cellular Component: microtubule cytoskeleton; kinetochore; nucleoplasm; cytoplasm; spindle midzone; nucleusMolecular Function: carbon-sulfur lyase activityBiological Process: positive regulation of cytokinesis; mitosis; cell division; metabolic process; ameboidal cell migration; centric heterochromatin formation; regulation of chromosome organization and biogenesis
