The CENPV Polyclonal Antibody (PAC02081) is a vital tool for researchers studying the Centromere Protein V (CENPV), a protein involved in chromosome segregation during cell division. This antibody, generated in rabbits, exhibits high specificity towards human samples and has been validated for use in Western blotting applications. By targeting the CENPV protein, this antibody allows for the precise detection and analysis of CENPV expression in a variety of cell types, making it an essential asset for investigations in cell biology and cancer research.
CENPV is a key player in ensuring the accurate distribution of chromosomes during mitosis, and its dysregulation can lead to chromosomal instability and contribute to the development of cancer. By delving into the functions of CENPV, researchers can gain valuable insights into the mechanisms underlying oncogenesis and potentially identify new therapeutic targets for cancer treatment. The CENPV Polyclonal Antibody is instrumental in unlocking the secrets of this important protein and advancing our understanding of its role in health and disease.
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO20281(CENPV Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
Gel: 10%SDS-PAGE, Lysate: 40 µg, Lane 1-4: Raji cells, Jurkat cells, 293T cells, hela cells, Primary antibody: PACO20281(CENPV Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO20281(CENPV Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
CENPV orthologs were detected in a range of species, including plants, but the proline-rich region was detected only in primates, rodents, birds, and amphibians, and long proline stretches were found only in primates. Northern blot analysis showed ubiquitous Cenpv expression in mouse tissues, with highest expression in testis, followed by small intestine, brain, and kidney. Immunofluorescence microscopy of mouse fibroblasts revealed colocalization of Cenpv with stabilized cytoplasmic microtubules. Western blot analysis of Ramos B cells detectedCENPV at an apparent molecular mass of 40 kD.
Synonyms:
centromere protein V
UniProt Protein Function:
CENPV: Required for distribution of pericentromeric heterochromatin in interphase nuclei and for centromere formation and organization, chromosome alignment and cytokinesis. Belongs to the gfa family. 3 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 17p11.2Cellular Component: microtubule cytoskeleton; kinetochore; nucleoplasm; cytoplasm; spindle midzone; nucleusMolecular Function: carbon-sulfur lyase activityBiological Process: positive regulation of cytokinesis; mitosis; cell division; metabolic process; ameboidal cell migration; centric heterochromatin formation; regulation of chromosome organization and biogenesis
