The CFAP44 Antibody (CAB14539) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, specifically targets CFAP44 in human samples and is optimized for Western blot applications. By binding to the CFAP44 protein, this antibody allows for accurate detection and analysis in various cell types, making it an essential component for studies in cell biology and ciliopathies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CFAP44 Antibody
SKU:
CAB14539
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
WDR52, SPGF20, CFAP44
Positive Sample:
OVCAR3, 22Rv1
Cellular Localization:
Cell Projection, Cilium, Flagellum.
Calculated MW:
214kDa
Observed MW:
112kDa
Enables peptidase activity. Involved in sperm axoneme assembly. Acts upstream of or within microtubule cytoskeleton organization. Predicted to be located in cytoplasm; cytoskeleton; and motile cilium. Implicated in spermatogenic failure 20.
Purification Method
Affinity purification
Gene ID
55779
RRID
AB_2761414
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CFAP44 Rabbit pAb (CAB14539) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
