Description
CFL1/CFL2 (Ab-88) Antibody (PACO23593)
The CFL1/CFL2 Polyclonal Antibody (AB-88) is a vital tool for researchers studying CFL1/CFL2, two closely related proteins involved in actin cytoskeleton regulation and cellular processes such as migration and contraction. This antibody, developed using a rabbit host, demonstrates high reactivity with human, mouse, and rat samples, making it versatile for a wide range of experimental applications. Validated for use in Western blotting and immunofluorescence, it specifically targets CFL1/CFL2 proteins, allowing for precise detection and analysis in different cell types.CFL1 and CFL2 are crucial players in cell motility, with CFL1 being an actin-severing protein and CFL2 functioning as an actin-binding protein.
Their roles in cytoskeleton dynamics have implications in various physiological and pathological processes, including cancer metastasis and neuronal development. By studying the expression and function of CFL1/CFL2, researchers can gain insights into cellular mechanisms and potentially identify therapeutic targets for diseases related to cytoskeletal defects. The CFL1/CFL2 Polyclonal Antibody (AB-88) is a valuable resource for investigations in cell biology, cancer biology, and neuroscience, offering researchers a precise and reliable tool for exploring the functions and interactions of CFL1 and CFL2 proteins in a variety of experimental settings.
| Antibody Name: | CFL1/CFL2 (Ab-88) Antibody (PACO23593) |
| Antibody SKU: | PACO23593 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Peptide sequence around aa. 86~90 (A-T-Y-E-T) derived from Human cofilin1/cofilin2. |
| Form: | Liquid |
| Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from MDA cells using cofilin1/cofilin2(Ab-88) Antibody. |
 | Immunofluorescence staining of methanol-fixed Hela cells using cofilin1/cofilin2(Ab-88) Antibody. |
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin1/cofilin2(Ab-88) Antibody(left) or the same antibody preincubated with blocking peptide(right). |
| Background: | Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. |
| Synonyms: | Cofilin; non-muscle isoform 18 kDa phosphoprotein; |
| UniProt Protein Function: | Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Cytoskeletal Chromosomal Location of Human Ortholog: 11q13 Cellular Component: cortical actin cytoskeleton; cytoplasm; extracellular space; focal adhesion; intercellular junction; membrane; nuclear matrix; nucleus; vesicle Molecular Function:actin binding; protein binding Biological Process: actin cytoskeleton organization and biogenesis; actin filament depolymerization; axon guidance; blood coagulation; cytokinesis after mitosis; cytoskeleton organization and biogenesis; ephrin receptor signaling pathway; establishment of cell polarity; innate immune response; negative regulation of apoptosis; neural crest cell migration; neural fold formation; platelet activation; platelet degranulation; positive regulation of actin filament depolymerization; protein amino acid phosphorylation; regulation of cell morphogenesis; response to amino acid stimulus; response to virus; Rho protein signal transduction |
| NCBI Summary: | The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004] |
| UniProt Code: | P23528 |
| NCBI GenInfo Identifier: | 116848 |
| NCBI Gene ID: | 1072 |
| NCBI Accession: | P23528.3 |
| UniProt Secondary Accession: | P23528,Q53Y87, Q9UCA2, B3KUQ1, |
| UniProt Related Accession: | P23528 |
| Molecular Weight: | 18,502 Da |
| NCBI Full Name: | Cofilin-1 |
| NCBI Synonym Full Names: | cofilin 1 |
| NCBI Official Symbol: | CFL1Â Â |
| NCBI Official Synonym Symbols: | CFL; cofilin; HEL-S-15Â Â |
| NCBI Protein Information: | cofilin-1 |
| UniProt Protein Name: | Cofilin-1 |
| UniProt Synonym Protein Names: | 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform |
| Protein Family: | Cofilin |
| UniProt Gene Name: | CFL1Â Â |
| UniProt Entry Name: | COF1_HUMAN |
| Antibodies |
| CFL1/CFL2 Antibody (PACO00619) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
