The cFLIP Antibody (CAB2555) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting CFLAR in human samples and is validated for use in Western blot applications. By binding to the CFLAR protein, this antibody allows for the precise detection and analysis of CFLAR expression in various cell types.CFLAR, also known as FLIP, plays a crucial role in modulating cell death pathways and maintaining cellular homeostasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
cFLIP Antibody
SKU:
CAB2555
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Jurkat, Mouse liver, Mouse heart, Rat liver, Rat heart
Cellular Localization:
Cytoplasm, Cytosol, Ripoptosome.
Calculated MW:
55kDa
Observed MW:
28kDa/55kDa
The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists.
Purification Method
Affinity purification
Gene ID
8837
RRID
AB_2764443
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using cFLIP Rabbit pAb (CAB2555) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of various lysates using cFLIP Rabbit pAb (CAB2555) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
