The p150 CAF1 Antibody (CAB2244) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for CHAF1A and has been validated for use in Western blot applications. It binds specifically to the CHAF1A protein, allowing for accurate detection and analysis in a variety of cell types.CHAF1A is known for its role in DNA replication and repair, as well as in maintaining genomic stability. Dysregulation of CHAF1A has been implicated in various diseases, including cancer, making it a promising target for further research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
p150 CAF1 Antibody
SKU:
CAB2244
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CAF1, P150, CAF-1, CAF1B, CAF1P150, p150 CAF1
Positive Sample:
HeLa, K-562
Cellular Localization:
Nucleus.
Calculated MW:
107kDa
Observed MW:
160kDa
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).
Purification Method
Affinity purification
Gene ID
10036
RRID
AB_2764247
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using p150 CAF1 Rabbit pAb (CAB2244) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
