The p150 CAF1 Antibody (CAB2244) is a high-quality antibody developed for reliable detection and analysis of target proteins. Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
p150 CAF1 Antibody
SKU:
CAB2244
Size:
100μL, 20μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CAF1, P150, CAF-1, CAF1B, CAF1P150, p150 CAF1
Positive Sample:
HeLa, K-562
Cellular Localization:
Nucleus.
Calculated MW:
107kDa
Observed MW:
160kDa
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).
Purification Method
Affinity purification
Gene ID
10036
RRID
AB_2764247
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using p150 CAF1 Rabbit pAb (CAB2244) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
