The Chicken IL-1β ELISA Kit is a specialized assay designed for the precise quantification of Interleukin-1 beta (IL-1β) levels in chicken biological samples.
IL-1β is a key pro-inflammatory cytokine that plays a crucial role in various cellular functions, including immune response modulation and inflammation regulation. This ELISA kit facilitates the accurate measurement of IL-1β concentrations in chicken samples, providing valuable insights into the inflammatory processes, immune reactions, and disease pathogenesis in chicken physiology. By quantifying IL-1β levels, researchers can gain a comprehensive understanding of its role in immune regulation and inflammatory conditions, leading to the development of targeted therapeutic interventions. Featuring exceptional sensitivity and specificity, the Chicken IL-1β ELISA Kit ensures precise and reproducible results, enabling researchers to explore the influence of IL-1β on chicken health and disease conditions. With robust performance and user-friendly protocols, this kit is an ideal choice for in-depth research in chicken immunology and inflammation studies.
Product Name:
Chicken IL-1beta ELISA Kit
SKU:
AEES00716
Target:
Chicken IL-1β
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Chicken IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Chicken IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Chicken IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Chicken IL-1β. You can calculate the concentration of Chicken IL-1β in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
89-102
86-98
Average (%)
99
94
93
1:4
Range (%)
88-100
81-92
86-99
Average (%)
95
87
93
1:8
Range (%)
92-103
83-96
85-101
Average (%)
97
88
92
1:16
Range (%)
89-104
82-95
86-98
Average (%)
95
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
95
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
94-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.17
39.23
147.57
21.41
41.13
148.14
Standard deviation
1.07
2.33
4.69
1.16
2.06
5.24
C V (%)
5.3
5.94
3.18
5.42
5.01
3.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100 μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Chicken IL-1β concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Chicken IL-1β in samples. No significant cross-reactivity or interference between Chicken IL-1β and analogues was observed.
