This kit is comprised by HRP conjugate, other reagents and ELISA Microtiter plate pre-coated with recombinant chicken mycoplasma gallisepticum (MG) protein. Apply the principle of enzyme-linked immunoassay (ELISA) to detect MG antibody in serum of chicken. During the experiment, add control serum and samples into the ELISA Microtiter plate. If MG antibodies exist in the samples, it will be bound with the recombinant protein on the microtiter plate after incubation. Then wash the plate to remove unbound antibodies and other components, add the HRP conjugate to specifically bind with the compound of antibody and antigen on the microtiter plate. The unbound HRP conjugate will be removed by washing. Substrate Reagent is added into the well, it will react with the enzyme and the products become blue. The color shade is positive correlation with antibody levels in the samples. At last, end the reaction by adding stop solution to produce a yellow product. Measure the absorbance value of each well by using a Microplate Reader with 450 nm wavelength, then we can know whether there are chicken MG antibody in the samples.
