The Chk1 Polyclonal Antibody (CAB21348) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is specifically designed for use in Western blotting and immunohistochemistry applications, providing reliable and accurate detection of CHK1 protein levels in human samples.CHK1 is a crucial protein in maintaining genomic stability and cell survival in response to DNA damage, making it a popular target in cancer research and drug development. Its role in cell cycle regulation and DNA repair pathways has garnered significant interest in understanding its function in tumorigenesis and potential therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Chk1 Polyclonal Antibody
SKU:
CAB21348
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
1111
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Chk1 Rabbit pAb (CAB21348) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer:3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: Q60s.
